ABSTRACT
Glucose and lipid metabolism is the most fundamental metabolic activity of higher organisms. This process is affected by both genetic polymorphisms and environmental factors. Excessive uptake and accumulation of lipids lead to obesity and disorder of glucose metabolic homeostasis characterized by insulin resistance and hyperglycemia, suggesting that the cross-regulation between lipid and glucose metabolism happens precisely at organ, cellular and molecular levels by known mechanisms. Adenine nucleotides and their metabolites have emerged as mediators in the mutual regulation of glucose and lipid metabolism. This review summarizes the roles of purinergic signaling induced by fatty acids in glucose metabolism and the development of type 2 diabetes.
Subject(s)
Humans , Adenine Nucleotides , Diabetes Mellitus, Type 2 , Glucose , Homeostasis , Insulin Resistance , Lipid MetabolismABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of ischemia postconditioning (IPC) on ischemia-reperfusion (I/R) injury and associated reperfusion injury salvage kinase (RISK) signal transduction pathways changes in isolated mouse hearts.</p><p><b>METHODS</b>Langendorff perfused C57/BL mouse hearts were divided to 6 groups: (1) control: 30 min global ischemia and 2 h reperfusion (I/R); (2) IPC with 3 episodes, IPC with 3 episodes of 10 s of ischemia and 10 s reperfusion after 30 min ischemia and before 2 h reperfusion; (3) IPC with 6 episodes, IPC with six episodes of 10 s of ischemia and 10 s reperfusion after 30 min ischemia and before 2 h reperfusion; (4) delayed IPC, IPC with 3 episodes of 10 s of ischemia and 10 s reperfusion after 30 min ischemia and at one minute after reperfusion; (5) IPC + ERK1/2 inhibitor PD98059 (10(-5) mol/L for 15 min); (6) I/R + ERK1/2 inhibitor PD98059 (10(-5) mol/L for 15 min). The effects of IPC on hemodynamics, coronary artery flow, creatine kinase (CK) and lactate dehydrogenase (LDH) release, myocardial SOD, MDA, phospho-protein kinase (P-ERK1/2) and phospho-protein kinase B (P-Akt) as well as myocardial infarction size were measured.</p><p><b>RESULTS</b>IPC with 3 episodes and IPC with 6 episodes significantly and equally improved myocardial function, increased myocardial SOD, reduced CK and LDH release and myocardial infarction size compared with IR group (all P < 0.01) while these parameters were similar between I/R hearts and delayed IPC hearts. IPC significantly increased myocardial ERK1/2 phosphorylation, PD98059 inhibited the phosphorylation of ERK1/2 and abolished the cardioprotective effects induced by IPC.</p><p><b>CONCLUSIONS</b>IPC obviously attenuated I/R injury in isolated mouse hearts, the cardioprotection of IPC was not enhanced because of increasing of IPC episodes and disappeared in delayed IPC. The cardioprotective effects of IPC were mediated through ERK1/2-MAPK signal transduction pathway.</p>
Subject(s)
Animals , Mice , Disease Models, Animal , Ischemic Preconditioning, Myocardial , Mice, Inbred C57BL , Mitogen-Activated Protein Kinases , Metabolism , Myocardial Ischemia , Metabolism , Therapeutics , Myocardial Reperfusion Injury , Metabolism , Therapeutics , Signal TransductionABSTRACT
<p><b>OBJECTIVES</b>To study whether Chinese Medicine Yiqihuoxuetang(YQHXT) could inhibit antisperm antibodies in infertile men, and to explore the therapeutical mechanism of YQHXT.</p><p><b>METHODS</b>Thirty infertile men with antisperm antibodies took YQHXT continuously for 60 days. Indirect immuno-fluorescence technique (IFT) was used to detect the levels of CD3, CD4, CD8 and CD4/CD8 ratio before and after treatment.</p><p><b>RESULTS</b>CD4 value and CD4/CD8 ratio after treatment were significantly lower than before treatment (P < 0.05); CD8 value became significantly higher(P < 0.05).</p><p><b>CONCLUSIONS</b>The results indicated that YQHXT could inhibit antisperm antibodies by keeping the balance of T-lymphocyte subpopulation in immunoinfertile men.</p>
Subject(s)
Adult , Humans , Male , Autoantibodies , Allergy and Immunology , CD4-CD8 Ratio , Drugs, Chinese Herbal , Pharmacology , Infertility, Male , Allergy and Immunology , Pathology , T-Lymphocyte SubsetsABSTRACT
Scleroglucan produced by Sclerotium spp. is a non-ionic polysacchari de biopolymer which has excellent viscosifying power in a wide variety of reservoi r brines snows high shear resistance and possesses good thermal stability. This article reviews the production of Scleroglucan , the influence of the conditions to the fermentation, and the application in the oil field.